448 Denny Hall
Box 353100
University of Washington
Seattle, Washington, 98195
Laboratory: (206) 616-9369
email: endolab.washington.edu

last updated: October 14, 2003
contact webmaster: csde.washington.edu

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 Welcome to the Biological Anthropology and Biodemography Laboratory E1G.File.Predict Program.

This program adjusts urinary E1G concentrations for non-parallelism exhibited by the 3F11 E1G enzyme immunoassay.

OConnor KA., Brindle E, Shofer JB, Miller RC, Klein NA, Soules MR, Campbell KL, Mar C, Handcock MS (2004). Statistical correction for non-parallelism in a urinary enzyme immunoassay. Journal of Immunoassay and Immunochemistry 25 (3): 259-278.
[ Full Text ]

Input File and Information

  1. The required input file format is a tab-delimited or space-delimited ascii text file without a header.
  2. The first column in the file must be the urine volume.
    • Volume units must be either microL/well OR dilution units (e.g. 20 microL/well or 2 for a 1:2 dilution). Click the appropriate radio button to indicate which units you used in your input file.
  3. The second column should be the dilution corrected E1G concentration.
    • Dilution-corrected-E1G units must be either nmol/L * dilution or pg/mL * dilution -- click the appropriate radio button to indicate which units you used in your input file.
  4. Specify the standard volume OR dilution level that you wish to have your E1G values corrected to. For example, to correct all your specimens to a 1:5 standard dilution, enter either 8 microL/well OR 5 for a 1:5 dilution.
    • Click the radio button to indicate the units of the standard (dilution or microL/well).
  5. Specify the alpha level for the confidence interval for the E1G values (for example, 0.05).

Output

The output file will be in the same units as your input file and the standard will be listed in the same units as the input of the standard volume.

The output file will be space-delimited ascii text with six columns. It will have a header.

  • The first two columns will contain the volume and dilution-corrected-E1G from the input file, respectively.
  • The third column will contain the standard (which is the same for all values in the file).
  • The fourth column will contain the dilution-corrected-E1G prediction (adjustment).
  • The fifth and sixth columns will have the lower and upper bound of a (1-alpha) level confidence interval for the predicted dilution-corrected-E1G concentration.

If you have any questions, please contact Kathleen O'Connor at oconnork@u.washington.edu.

Select your input file:

vol.units:


dcE1G.units:


standard:

std.units:


alpha:

After hitting the 'Submit Query' button, your browser window will go blank for a few moments and then refresh. You may then scroll down to see the download link.