448 Denny Hall
Box 353100
University of Washington
Seattle, Washington, 98195
Laboratory: (206) 616-9369
email: endolab.washington.edu

last updated: October 14, 2003
contact webmaster: csde.washington.edu

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 Welcome to the Biological Anthropology and Biodemography Laboratory E1G.Predict Program.

This program adjusts urinary E1G concentrations for non-parallelism exhibited by the 3F11 E1G enzyme immunoassay.

OConnor KA., Brindle E, Shofer JB, Miller RC, Klein NA, Soules MR, Campbell KL, Mar C, Handcock MS (2004). Statistical correction for non-parallelism in a urinary enzyme immunoassay. Journal of Immunoassay and Immunochemistry 25 (3): 259-278.
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The E1G.Predict program requires
1) the urine μL/well in log units at which the specimen was assayed
2) dilution adjusted E1G concentration in log units (Please note: assay results in mass units [i.e. pg/mL] may be substituted where results in nmol/L are specified.)
3) the standard urine μL/well in log units to which the concentration is to be adjusted to correct for non-parallelism. The standard urine μL/well should correspond to the most commonly used dilution for the study (i.e. 8μL/well if most specimens are assayed at a 1:5 dilution).
The program output gives the E1G concentration adjusted for dilution and corrected for non-parallelism, the standard deviation of the adjusted value, and 90% CI, all reported in log units.

Please enter the urine volume in log(μL/well) units:

Please enter the dilution corrected E1G concentration in log(nmol/L * dilution) units:

Please enter the standard urine volume, in log(μL/well) units, to adjust non-parallelism to:

After hitting the Submit Query button, you may need to scroll down to see the program results.