Biodemography Services

Methods

Assays

The following is a list of the in-house assays routinely employed in the Biodemography Lab. Many of these assays were developed in our lab specifically for population-level biomarker research. We are working continuously to expand this list of in-house assays to meet the demand for new biomarker tools.

In addition to these, our lab has the capacity to measure many biomarkers using commercial assay kits, including kits based on enzyme, fluorescence, time-resolved fluorescence, or luminescence assay formats. Commercial kits, while often too expensive for very large projects, can be very useful for pilot work or for smaller projects.

Publications

  • Fujita M, Brindle E, Shell-Duncan B, Shofer J, O’Connor KA. (submitted) Retinol-binding protein stability in dried blood spots. Clinical Chemistry. http://csde.washington.edu/downloads/06-02.pdf

  • Snipes,SA, Thompson B, O’Connor KA, Godina R, Ibarra G (revision submitted) Anthropological and psychological merge: Design of a stress measure for Mexican farmworkers. Culture, Medicine and Psychiatry.

  • O’Connor, KA, Holman DJ, Brindle E, Miller RC, Barsom SH, Wood JW (under revision) Pregnancy-related sickness in rural Bangladesh: Symptoms and their links with reproductive hormones. Obstetrics and Gynecology. (See Also: Center for the Study of Demography and Ecology Working Paper 04-07, http://csde.washington.edu/downloads/04-07.pdf).

  • Mitchell ES, O’Connor KA, Taylor HJ, Brindle E, Smith-DiJulio K, Mariella A, Tao EY, Woods NF. (under revision) Urinary FSH and Estrone Patterns During the Menopausal Transition: The Seattle Midlife Women’s Health Study. Climacteric.

  • Brindle E, Miller RC, Shofer JB, Klein NA, Soules MR, O’Connor KA. (in press) Urinary beta-luteinizing hormone and beta-follicle stimulating hormone immunoenzymometric assays for population research. Clinical Biochemistry. http://dx.doi.org/10.1016/j.clinbiochem.2006.08.009

  • Ferrell RJ, O’Connor KA, Rodriguez G, Holman DJ, Brindle E, Miller RC, Simon J, Mansfield PK, Wood JW, Weinstein M. (in press) Monitoring reproductive aging in a 5-year prospective study: aggregate and individual changes in luteinizing hormone (LH) and follicle-stimulating hormone (FSH) with age. Menopause. Abstract

  • Ferrell RJ, Simon JA, Pincus SM, Holman DJ, O’Connor KA, Rodriguez G, Weinstein M. (in press) The length of perimenopausal menstrual cycles increases later and to a greater degree than previously reported. Fertility and Sterility. http://dx.doi.org/10.1016/j.fertnstert.2006.01.045

  • O’Connor KA, Brindle E, Miller RC, Shofer JB, Ferrell RJ, Klein NA, Soules MR, Holman DJ, Mansfield PK, Wood JW. (2006) Ovulation detection methods for urinary hormones: Precision, daily and intermittent sampling, and a combined hierarchical method, Human Reproduction 21(6):1442-1452. http://humrep.oxfordjournals.org/cgi/content/full/21/6/1442

  • Holman DJ, Grimes MA, Achterberg JT, Brindle E, O’Connor KA. (2006). The distribution of postpartum amenorrhea in rural Bangladeshi women. American Journal of Physical Anthropology 129(4):609-619. http://www3.interscience.wiley.com/cgi-bin/fulltext/112211588/PDFSTART

  • Ferrell RJ, O’Connor KA, Rodriguez G, Gorrindo T, Holman DJ, Brindle E, Miller RC, Schechter DE, Korshalla L, Simon J, Mansfield PK, Wood JW, Weinstein M. (2005) Monitoring reproductive aging in a five year prospective study: aggregate and individual changes in steroid hormones and menstrual cycle lengths with age. Menopause 12(5):567-577. http://csde.washington.edu/downloads/Ferrell_et_al_05_Menopause.pdf

  • Holman DJ, Grimes MA, Brindle E, O’Connor KA. (2004) Hormonal correlates for the initiation of breastfeeding in Bangladeshi women. Hormones and Behavior 46(4):382-391. http://dx.doi.org/10.1016/j.yhbeh.2004.04.001

  • O’Connor KA, Brindle E, Shofer JB, Miller RC, Klein NA, Soules MR, Campbell KL, Mar C, Handcock MS. (2004) Statistical correction for non-parallelism in a urinary enzyme immunoassay. Journal of Immunoassay and Immunochemistry 25(3):259-278. http://depts.washington.edu/endolab/JII_Sept_2004_Paper.pdf

  • Miller RC, Brindle E, Holman DJ, Shofer JB, Klein NA, Soules MR, O’Connor KA (2004) Comparison of specific gravity and creatinine methods for normalizing urinary reproductive hormone concentrations. Clinical Chemistry 50(5):924-32. http://www.clinchem.org/cgi/reprint/50/5/924

  • O’Connor KA, Brindle E, Holman DJ, Klein NA, Soules MR, Campbell KL, Kohen F, Munro CJ, Shofer J, Lasley WL, Wood JW. (2003) Urinary estrone conjugate and pregnanediol-3-glucuronide enzyme-immunoassays for population research. Clinical Chemistry 49(7):1139-1148. http://www.clinchem.org/cgi/reprint/49/7/1139

  • McConnell HJ, O’Connor KA, Brindle E, Williams NI (2002) Validity of methods for analyzing urinary steroid data to detect ovulation in athletes. Medicine & Science in Sports & Exercise 34(11):1836-1844. Abstract

  • O’Connor KA, Holman DJ, Wood JW (2001) Menstrual cycle variability and the perimenopause. American Journal of Human Biology 13(4):465-478. http://www3.interscience.wiley.com/cgi-bin/abstract/82003865/ABSTRACT

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